List of culture media used in microbiology with their uses
Many types of bacterial growth media are used to culture (grow) microbes
in the laboratory. Here's a summary of defined, complex, selective and
differential media.
Read more:
Microbiology notes
Media
|
Uses
|
· Alkaline Peptone Water
|
Enrichment media for Vibrio cholerae
|
· Alkaline Salt Transport Medium
·
Taurocholate Peptone Transport Medium
|
Transport media for diarrheal diseases suspected of
being caused by V. cholerae
|
· Anaerobic Media
|
Liquid media by addition of
·
Glucose (0.5 % to 1 %)
·
Ascorbic Acid (0.1 %)
·
Cysteine (0.1 %)
·
Sodium Merceptoacetate (0.1 %)
·
Thioglycollate (0.1 %)
·
Particles of cooked meat broth
|
· Bile Salt Agar
·
Thiosulphate Citrate Bile Salts-Sucrose Agar (TCBS)
·
Monsur’s Tellurite Taurocholate Gelatin Agar
|
Selective media for V. cholerae
|
· Bile Esculin Agar (Contains 40% Bile)
|
Selective media for Enterococcus species (Black
coloration of the medium)
|
· Blood Agar
|
· Enriched media (Supports
the growth of fastidious organisms, e.g. Streptococcus)
·
Indicator media to show hemolytic properties of certain organisms (Staphylococcus
aureus: β-Hemolytic; Streptococcus pneumonia and S
viridans: α-Hemolytic; Enterococcus: Non-Hemolytic)
|
· Bordet-Gengou Agar
·
Charcoal Blood Agar
·
Regan-Lowe Medium (Charcoal Agar with blood, cephalexin and Amphotericin B)
|
Isolation of Bordetella pertussis
|
· Brain Heart Infusion Broth
|
Used in blood culture bottles (both adult and
pediatric patients)
|
· Buffered Charcoal Yeast Agar (BCYA)
· Feeley Gorman Agar
|
Specialized media for isolation of Legionella
|
· Campylobacter Thioglycollate Broth
|
Selective holding media for recovery of Campylobacter
species
|
· Castaneda Medium
|
Biphasic medium for the isolation of Brucella
|
· Cefoxitin Cycloserine Fructose Agar
(CCFA)
·
Cefoxitin Cycloserine Egg-Yolk Agar (CCEY)
|
Selective media for isolation of Clostridium
difficile form suspected cases of pseudomembranous
colitis/antibiotic-associated diaeehea
|
· Cefsulodin-Irgasan-Novobiocin medium (CIN Medium)
|
Selective media for Yersinia (and may be used
for Aeromonas also)
|
· Columbia-Colistin Nalidixic Acid agar
(CNA Agar)
|
Selective media for the isolation of Gram-positive
cocci
|
· Cooked meat broth
·
Nutrient agar slopes
·
Semisolid nutrient agar stabs
·
Heated blood agar slopes
|
In general, used for preservation and storage of
bacterial cultures
|
· Crystal violet blood agar
|
Selective media for Streptococcus pyogenes
|
· Cysteine Lactose Electrolyte Deficient Media (CLED Media)
|
Most commonly used media for culturing urine samples
|
· Egg Saline Medium
|
Preservation of cultures of Gram-negative bacilli
|
· Egg Yolk Agar
|
Detection of lipase and lecithinase activity of Clostridium
species
|
· Ellner’s Medium
·
Medium of Duncan and Strong
·
Medium of Phillips
·
Alkaline Egg Medium
|
Specialized media to induce sporulation in Clostridium
|
· Fildes Blood-Digest Agar and Broth
· Levinthal’s Agar
|
Enriched media for recovery of Haemophilus influenzae
|
· Firm Agar (4% to 6% Agar)
|
Prevents swarming of Proteus mirabilis, P.
vulgaris and Clostridium tetani
|
· Fletcher’s Agar
·
Ellinghausen and McCullough Medium
· Ellinghausen-McCullough-Johnson-Harris (EMJH) Media
|
Solid media for isolation of Leptospira
|
· Glycerol Saline Transport Medium
|
Transport stool specimen for typhoid bacilli
|
· Heated Blood Agar/Chocolate Agar
|
Growth of fastidious organisms (E.g. Hemophilus
influenzae, Neisseria
gonorrhoeae and S. pneumoniae
|
· Hoyle’s Tellurite Lysed Blood Agar
·
Tinsdale Medium
|
Selective media for isolation of Corynebacterium
from throat swabs
|
· Loeffler Serum Slope
|
Stimulation of metachromatic granules in Corynebacterium
diphtheriae
|
· Lowenstein-Jensen Medium
·
Middlebrook Media
|
Selective media for isolation of Mycobacterium
tuberculosis from sputum and other samples
|
· MacConkey Agar
|
Differential media for Enterobacteriacaeae (i.e.,
lactose fermenting and non-lactose fermenting)
|
· MacConkey Bile Salt Lactose Agar
·
Brilliant MacConkey Agar
·
Leifson’s Deoxycholate-Citrate Agar (DCA)
·
Wilson and Blair’s Brilliant Green Bismuth- Sulphite Agar (BBSA)
·
Taylor’s Xylose Lysine Deoxycholate Agar (XLD)
· Hektoen Enteric Agar
· Salmonella–Shigella Agar
|
Differential and media for isolation of Salmonella
and Shigella from stool specimens
|
· Mannitol Salt Agar
|
Selective and indicator media for S. aureus
|
· Modified Barbour Stoenner Kelly medium (BSK)
|
Specialized media for Borrelia burgdorferi
|
· Modified Korthoff’s Medium
|
Liquid media for isolation of Leptospira
|
· Modified New York City Medium (contains colistin, lincomycin, trimethoprim,
amphotericin B)
|
Selective media for Neisseria gonorrhoeae
|
· Mueller-Hinton Agar
|
Performing antimicrobial susceptibility for bacteria
|
· Nutrient Agar (1% to 2% Agar)
|
Basal media in microbiology
Supports the
growth of all non-fastidious organisms
|
· Non-Nutrient Agar
|
Cultivation of parasites (e.g., Acanthamoeba)
|
· Peptone Water
|
· Basal media for
preparation for carbohydrate fermentation media
·
To ascertain whether a bacteria is motile or non-motile
· Basis for Indole test
|
· Phenol-Red Egg Yolk Polymyxin Agar
|
Selective media for isolation of Bacillus cereus
from food, feces, and vomitus
|
· Pike’s Media
|
Preservation of S. pyogenes, pneumococci, and
Hemophilus influenzae in nose and
throat swabs
|
· Polymyxin B-lysozyme-EDTA-Thallous
Acetate (PLET)
|
Selective media for isolation of Bacillus
anthracis from soil and other medium materials containing numerous spore
formers of other species
|
· Polymyxin B, Neomycin, Fusidic Acid
Media (PNF)
|
Selective media for S. pyogenes (or β-hemolytic Streptococcus)
|
· PPLO Medium (Contains Sterol)
|
Specialized media for Mycoplasma pneumoniae
|
· Pre-Reduced Anaerobically Sterilized
(PRAS) Media
|
Commercially available media for anaerobic organisms
|
· Proteose Peptone-Yeast Extraction
Broth
|
Media for carrying out biochemical tests for
anaerobes
|
· Requirements of X and V Factors
|
Isolation of Haemophilus influenzae
|
· Roswell Park Memorial Institute (RPMI)
1640 Medium
|
Cultivation of malarial parasites (i.e., Plasmodium)
|
· Robertson Cooked Meat Broth (RCMB)
|
· Growth of anaerobes
(e.g., Clostridium)
·
Maintaining stock cultures of anaerobic organisms
|
· Salt-Cooked Meat Broth (Cooked Meat
Broth with 10% NaCl)
|
Enrichment media for isolation of S. aureus
from heavily contaminated materials
|
· Semisolid Agar (0.05% to 0.1% Agar)
|
Prevents convection current and allows the growth of
anaerobic and micro-aerophilic organisms
|
· Skirrow’s Campylobacter Medium
(contains polymixin B, trimethoprim, vancomycin)
·
Preston Campylobacter Medium (contains polymixin B, rifampicin, trimethoprim)
·
Campy Blood Agar
·
CVA Medium (contains cefoperazone, vancomycin, amphotericin)
|
Selective media for Campylobacter jejuni
|
· Smith-Noguchi Medium
|
Cultivation of nonpathogenic treponemes (e.g.,
Reiter strain of Treponema phagedenis)
|
· Sorbitol MacConkey Agar
|
Isolation of verocytotoxin-producing
(enterohemorrhagic) E. coli of 0157 type (as it fails to ferment
D-sorbitol)
|
· Stuart Transport Media
·
Amies Transport Media
|
Maintaining the viability of gonococci on swabs
during transportation
|
· Tetrathionate Broth
·
Gram-Negative Broth
·
Selenite-F Broth
|
Enrichment media for isolation of Shigella
and Salmonella from stool samples
|
· Thayer-Martin Medium (contains
vancomycin, colistin, nystatin)
|
Selective media for Neisseria gonorrhoeae
|
· Thioglycollate Broth
·
Trypticase Soy Broth
|
All purpose enrichment broth for anaerobes, aerobes,
micro-aerophilic, and fastidious organisms
|
· Todd Hewitt Broth with Antibiotics
|
Selective and enrichment for Streptococccus
agalactiae in female genital specimens
|
· Triple Sugar Iron Agar (TSI) Medium
|
Differentiation of various members of
Enterobacteriaceae
|
· Wilkins-Chalgren Agar
|
Performing antimicrobial susceptibility of anaerobic
bacteria
|
Source:Microbiology notes
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