List of culture media used in microbiology with their uses
Many types of bacterial growth media are used to culture (grow) microbes in the laboratory. Here's a summary of defined, complex, selective and differential media.
Read more: Microbiology notes
Media
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Uses
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· Alkaline Peptone Water
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Enrichment media for Vibrio cholerae
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· Alkaline Salt Transport Medium
· Taurocholate Peptone Transport Medium
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Transport media for diarrheal diseases suspected of being caused by V. cholerae
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· Anaerobic Media
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Liquid media by addition of
· Glucose (0.5 % to 1 %)
· Ascorbic Acid (0.1 %)
· Cysteine (0.1 %)
· Sodium Merceptoacetate (0.1 %)
· Thioglycollate (0.1 %)
· Particles of cooked meat broth
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· Bile Salt Agar
· Thiosulphate Citrate Bile Salts-Sucrose Agar (TCBS)
· Monsur’s Tellurite Taurocholate Gelatin Agar
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Selective media for V. cholerae
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· Bile Esculin Agar (Contains 40% Bile)
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Selective media for Enterococcus species (Black coloration of the medium)
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· Blood Agar
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· Enriched media (Supports the growth of fastidious organisms, e.g. Streptococcus)
· Indicator media to show hemolytic properties of certain organisms (Staphylococcus aureus: β-Hemolytic; Streptococcus pneumonia and S viridans: α-Hemolytic; Enterococcus: Non-Hemolytic)
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· Bordet-Gengou Agar
· Charcoal Blood Agar
· Regan-Lowe Medium (Charcoal Agar with blood, cephalexin and Amphotericin B)
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Isolation of Bordetella pertussis
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· Brain Heart Infusion Broth
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Used in blood culture bottles (both adult and pediatric patients)
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· Buffered Charcoal Yeast Agar (BCYA)
· Feeley Gorman Agar
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Specialized media for isolation of Legionella
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· Campylobacter Thioglycollate Broth
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Selective holding media for recovery of Campylobacter species
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· Castaneda Medium
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Biphasic medium for the isolation of Brucella
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· Cefoxitin Cycloserine Fructose Agar (CCFA)
· Cefoxitin Cycloserine Egg-Yolk Agar (CCEY)
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Selective media for isolation of Clostridium difficile form suspected cases of pseudomembranous colitis/antibiotic-associated diaeehea
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· Cefsulodin-Irgasan-Novobiocin medium (CIN Medium)
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Selective media for Yersinia (and may be used for Aeromonas also)
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· Columbia-Colistin Nalidixic Acid agar (CNA Agar)
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Selective media for the isolation of Gram-positive cocci
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· Cooked meat broth
· Nutrient agar slopes
· Semisolid nutrient agar stabs
· Heated blood agar slopes
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In general, used for preservation and storage of bacterial cultures
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· Crystal violet blood agar
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Selective media for Streptococcus pyogenes
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· Cysteine Lactose Electrolyte Deficient Media (CLED Media)
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Most commonly used media for culturing urine samples
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· Egg Saline Medium
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Preservation of cultures of Gram-negative bacilli
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· Egg Yolk Agar
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Detection of lipase and lecithinase activity of Clostridium species
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· Ellner’s Medium
· Medium of Duncan and Strong
· Medium of Phillips
· Alkaline Egg Medium
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Specialized media to induce sporulation in Clostridium
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· Fildes Blood-Digest Agar and Broth
· Levinthal’s Agar
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Enriched media for recovery of Haemophilus influenzae
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· Firm Agar (4% to 6% Agar)
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Prevents swarming of Proteus mirabilis, P. vulgaris and Clostridium tetani
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· Fletcher’s Agar
· Ellinghausen and McCullough Medium
· Ellinghausen-McCullough-Johnson-Harris (EMJH) Media
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Solid media for isolation of Leptospira
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· Glycerol Saline Transport Medium
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Transport stool specimen for typhoid bacilli
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· Heated Blood Agar/Chocolate Agar
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Growth of fastidious organisms (E.g. Hemophilus influenzae, Neisseria gonorrhoeae and S. pneumoniae
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· Hoyle’s Tellurite Lysed Blood Agar
· Tinsdale Medium
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Selective media for isolation of Corynebacterium from throat swabs
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· Loeffler Serum Slope
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Stimulation of metachromatic granules in Corynebacterium diphtheriae
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· Lowenstein-Jensen Medium
· Middlebrook Media
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Selective media for isolation of Mycobacterium tuberculosis from sputum and other samples
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· MacConkey Agar
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Differential media for Enterobacteriacaeae (i.e., lactose fermenting and non-lactose fermenting)
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· MacConkey Bile Salt Lactose Agar
· Brilliant MacConkey Agar
· Leifson’s Deoxycholate-Citrate Agar (DCA)
· Wilson and Blair’s Brilliant Green Bismuth- Sulphite Agar (BBSA)
· Taylor’s Xylose Lysine Deoxycholate Agar (XLD)
· Hektoen Enteric Agar
· Salmonella–Shigella Agar
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Differential and media for isolation of Salmonella and Shigella from stool specimens
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· Mannitol Salt Agar
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Selective and indicator media for S. aureus
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· Modified Barbour Stoenner Kelly medium (BSK)
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Specialized media for Borrelia burgdorferi
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· Modified Korthoff’s Medium
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Liquid media for isolation of Leptospira
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· Modified New York City Medium (contains colistin, lincomycin, trimethoprim, amphotericin B)
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Selective media for Neisseria gonorrhoeae
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· Mueller-Hinton Agar
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Performing antimicrobial susceptibility for bacteria
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· Nutrient Agar (1% to 2% Agar)
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Basal media in microbiology
Supports the growth of all non-fastidious organisms
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· Non-Nutrient Agar
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Cultivation of parasites (e.g., Acanthamoeba)
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· Peptone Water
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· Basal media for preparation for carbohydrate fermentation media
· To ascertain whether a bacteria is motile or non-motile
· Basis for Indole test
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· Phenol-Red Egg Yolk Polymyxin Agar
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Selective media for isolation of Bacillus cereusfrom food, feces, and vomitus
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· Pike’s Media
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Preservation of S. pyogenes, pneumococci, andHemophilus influenzae in nose and throat swabs
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· Polymyxin B-lysozyme-EDTA-Thallous Acetate (PLET)
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Selective media for isolation of Bacillus anthracis from soil and other medium materials containing numerous spore formers of other species
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· Polymyxin B, Neomycin, Fusidic Acid Media (PNF)
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Selective media for S. pyogenes (or β-hemolytic Streptococcus)
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· PPLO Medium (Contains Sterol)
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Specialized media for Mycoplasma pneumoniae
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· Pre-Reduced Anaerobically Sterilized (PRAS) Media
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Commercially available media for anaerobic organisms
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· Proteose Peptone-Yeast Extraction Broth
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Media for carrying out biochemical tests for anaerobes
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· Requirements of X and V Factors
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Isolation of Haemophilus influenzae
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· Roswell Park Memorial Institute (RPMI) 1640 Medium
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Cultivation of malarial parasites (i.e., Plasmodium)
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· Robertson Cooked Meat Broth (RCMB)
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· Growth of anaerobes (e.g., Clostridium)
· Maintaining stock cultures of anaerobic organisms
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· Salt-Cooked Meat Broth (Cooked Meat Broth with 10% NaCl)
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Enrichment media for isolation of S. aureusfrom heavily contaminated materials
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· Semisolid Agar (0.05% to 0.1% Agar)
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Prevents convection current and allows the growth of anaerobic and micro-aerophilic organisms
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· Skirrow’s Campylobacter Medium (contains polymixin B, trimethoprim, vancomycin)
· Preston Campylobacter Medium (contains polymixin B, rifampicin, trimethoprim)
· Campy Blood Agar
· CVA Medium (contains cefoperazone, vancomycin, amphotericin)
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Selective media for Campylobacter jejuni
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· Smith-Noguchi Medium
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Cultivation of nonpathogenic treponemes (e.g., Reiter strain of Treponema phagedenis)
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· Sorbitol MacConkey Agar
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Isolation of verocytotoxin-producing (enterohemorrhagic) E. coli of 0157 type (as it fails to ferment D-sorbitol)
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· Stuart Transport Media
· Amies Transport Media
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Maintaining the viability of gonococci on swabs during transportation
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· Tetrathionate Broth
· Gram-Negative Broth
· Selenite-F Broth
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Enrichment media for isolation of Shigella and Salmonella from stool samples
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· Thayer-Martin Medium (contains vancomycin, colistin, nystatin)
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Selective media for Neisseria gonorrhoeae
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· Thioglycollate Broth
· Trypticase Soy Broth
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All purpose enrichment broth for anaerobes, aerobes, micro-aerophilic, and fastidious organisms
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· Todd Hewitt Broth with Antibiotics
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Selective and enrichment for Streptococccus agalactiae in female genital specimens
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· Triple Sugar Iron Agar (TSI) Medium
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Differentiation of various members of Enterobacteriaceae
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· Wilkins-Chalgren Agar
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Performing antimicrobial susceptibility of anaerobic bacteria
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Source:Microbiology notes
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